| Product Name | SensoLyte® 520 SARS - CoV - 2 Papain - like Protease/ De - ubiquitinase Activity Assay KitNEW |
| Size | 1 kit |
| Catalog # | AS-72263 |
| US$ | $511 |
SARS-Cov-2 Papain-like Protease (PLpro) is encoded as non-structural protein 3 (nsp3) and it is involved in the cleavage of the viral polyprotein. This enzyme hydrolyzes ubiquitin chains which are important in the inflammation process. It also removes interferon-stimulated gene 15 (ISG15) modifications from proteins to reverse antiviral responses. As an essential enzyme in the process of SARS-CoV-2 replication and infection of the host, PLpro has been an attractive drug target for research and development of inhibitors of coronavirus. The SensoLyte® 520 SARS-CoV-2 Papain-like Protease/ De-ubiquitinase Activity Assay Kit employs a fluorescence peptide substrate for the detection of de-ubiquitnation activity. When active Papain-like Protease cleaves the substrate, it results in an increase of bright green fluorescence at excitation/emission = 490 nm/520 nm. This assay can detect as low as 7.8 ng/mL active Papain-like Protease in the sample. This kit can be used to detect enzyme activity in purified enzyme preparations, biological samples and can also be applied for compound screening. Kit size: 100 assays (96-well plate) Fig 1: Inhibition of Papain-like protease activity by GRL0617 as measured with SensoLyte 520 SARS-CoV-2 Papain-like Protease/Deubiquitinase Activity Assay Kit. | |
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| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | Component C at -80 °C, Component A, B, D, E at -20 °C and Component A and B avoid light and moisture |
| References | 1. Freitas, BT. et al,ACS Infect Dis. 4, 0c00168 (2020). 2. Wu, C. et al, Acta Pharm Sin B. 10, 766 (2020).3. Klemm, T. et al, bioRxiv. 06, 160614 (2020).4. Mielech, AM. et al,J Virol. 89, 4907 (2015).5. Ratia, K. et al,Proc Natl Acad Sci U S A. 103, 5715 (2006).6. Dömling, A. et al,Chem. 6, 1283 (2020). |
