| Product Name | SensoLyte ® 520 HIV Protease Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-71147 |
| US$ | $480 |
The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor 488 (fluorophore) and QXL® 520 (quencher) for continuous measurement of enzyme activities.In the intact FRET peptide, the fluorescence of HiLyte Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte Fluor 488 is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte Fluor 488/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: HiLyte Fluor 488/QXL® 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) Assay buffer HIV protease inhibitor Fluorescence reference standard for calibration A detailed protocol Kit Size: 100 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) Poster |
| Product Citations | Omoruyi BE, et al. (2020) Inhibition of HIV-1 Protease by Carpobrotus edulis (L.). Evidence-Based Complementary and Alternative Medicine. 2020. 9648056. doi: 10.3389/fcell.2020.00608.Meng, J. et al. (2015). Screening of HIV-1 protease using a combination of an ultra-high-throughput fluorescent-based assay and RapidFire mass spectrometry. J Biomol Screen 20, 606. doi: 10.1177/1087057115570838.Lu, D. et al. (2010). Design, asymmetric synthesis, and evaluation of pseudosymmetric sulfoximine inhibitors against HIV-1 protease. Bioorg Med Chem 18, 2037. doi: 10.1016/j.bmc.2010.01.020.Wei, Y. et al. (2009). Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases. Bioorganic and Medicinal Chem 17, 3003. |
