| Product Name | SensoLyte ® 520 HCV Protease Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-71145 |
| US$ | $480 |
The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL® 520 (quencher) for a continuous measurement of enzyme activities.In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: 5-FAM/QXL® 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) Fluorescence reference standard for calibration A detailed protocol Kit Size: 100 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) Poster |
| Product Citations | Dultz G, et al. (2020) Extended interaction networks with HCV protease NS3-4A substrates explain the lack of adaptive capability against protease inhibitors. Journal of Biological Chemistry. 295(40):13862-74. doi: 10.1074/jbc.RA120.013898Pessoa, L. et al. (2016) Development of a rapid phenotypic test for HCV protease inhibitors with potential use in clinical decisions. Genetics & Molecular Biology, 39, 3, 358-364Furuta, A. et al. (2014). Identification and biochemical characterization of Halisulfate 3 and Suvanine as novel inhibitors of Hepatitis C Virus NS3 Helicase from a marine sponge. Marine drugs 12, 462.Dine R, et al. (2011). HCV-NS3/4A Protease inhibitory iridoid glucosides and dimeric foliamenthoic ccid derivatives from Anarrhinum orientale. J. Nat. Prod 74, 943.Salim, MTA. et al. (2011). Potent and selective inhibition of hepatitis C virus replication by novel phenanthridinone derivatives. Biochem Biophys Res Commun 415, 714.Ma, C. et al. (2009). HCV Protease Inhibitory, Cytotoxic and Apoptosis-Inducing Effects of Oleanolic Acid Derivatives. J Pharm Pharmaceut Sci 12, 243.Phuong, DT. et al. (2009). Inhibitory effects of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. Phytother. Res. 23, 582.Wang, S-Y. et al. (2009). Bioactivity-guided screening identifies pheophytin a as a potent anti-hepatitis C virus compound from Lonicera hypoglauca Miq. BBRC 385, 230.Wei Y, et al. (2009). Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases. Bioorg Med Chem. 17, 3003.Ma, Y. et al. (2008). NS3 helicase domains involved in infectious intracellular Hepatitis C Virus particle assembly. J. Virol. 82, 7624.Ma, C. et al. (2007). Triterpenes from Cynomorium songaricium analysis of HCV protease inhibitory activity, quantification, and content change under the influence of heating. J Nat Med 63, 9. |
