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Anaspec/SensoLyte ® AFC Thrombin Activity Assay Kit *Fluorimetric*/1 kit/AS-72130
| Product Name | SensoLyte ® AFC Thrombin Activity Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72130 |
| US$ | $456 |
Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation.Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.The SensoLyte® AFC Thrombin Assay Kit is optimized for screening of enzyme inhibitors.This kit contains a fluorogenic substrate which shows high reactivity and low background.Thrombin cleaves the substrate resulting in the release of the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore.Fluorescence can be monitored at excitation/emission= 380 nm/500 nm.The assays are performed in a convenient 96-well or 384-well microplate format. Kit size: 100 assays. | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Product Citations | Ambadapadi, S. et al. (2015) Reactive Center Loop (RCL) peptides derived from serpins display independent coagulation and immune modulating activities J. Biol. Chem. 291 2874-2887doi: 10.1074/jbc.M115.704841Nair, DG. et al. (2014). Interactions of some commonly used drugs with Human α-thrombin. J Biomol Struct Dyn doi: 10.1080/07391102.2014.923329.Wendeler, M. et al. (2014). Process-scale purification and analytical characterization of highly gamma-carboxylated recombinant human prothrombin. J Chromatogr A 1325, 171.Yang, H. et al. (2013). Interleukin-1 promotes coagulation, which Is necessary for protective immunity in the lung against Streptococcus pneumoniae infection. J Infect Dis 207, 50. doi: 10.1093/infdis/jis651.Sasaki, T. et al. (2012). Intravenous and oral administrations of DD2 [7-Amino-2-(sulfanylmethyl)heptanoic acid] produce thrombolysis through inhibition of plasma TAFIa in rats with tissue factor-induced microthrombosis. Thrombosis Res 130, e222. doi:10.1038/jid.2012.204Lim, HA. et al. (2011). Novel agmatine and agmatine-like peptidomimetic inhibitors of the West Nile Virus NS2B/NS3 serine protease. Eur J Med Chem 10.1016/j.ejmech.2011.04.055. |
