| Product Name | SensoLyte ® 520 Thrombin Activity Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72129 |
| US$ | $526 |
Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation.Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity.This kit contains a novel internally quenched 5-FAM/QXL® 520 FRET substrate for thrombin.Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm.The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors. Kit size: 100 assays. | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
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