| Product Name | SensoLyte ® 520 Renin Assay Kit*Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72040 |
| US$ | $611 |
Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme).Angiotensin II constricts blood vessels leading to increased blood pressure.It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease. The SensoLyte® 520 Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL®520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL®520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay and can detect 0.8 ng/ml renin. The kit contains: 5-FAM/QXL®520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) Assay buffer Human recombinant renin Renin inhibitor Fluorescence reference standard for calibration A detailed protocol 100 assays (96-well plate)
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| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) Poster |
| Storage | -20°C |
| Product Citations | Schmiedt, CW. et al. (2012). Bilateral renal ischemia as a model of acute kidney injury in cats. Res Vet Sc doi.org/10.1016/j.rvsc.2011.12.004.Fujita, M. et al. (2011). Antihypertensive Effects of Continuous Oral Administration of Nattokinase and Its Fragments in Spontaneously Hypertensive Rats. Biol. Pharm. Bull. 34;1696.May, PC. et al. (2011). Robust central reduction of amyloid-β in humans with an orally available, non-peptidic β-secretase inhibitor. J Neurosc 31, 16507. doi: 10.1523/JNEUROSCI.3647-11.2011Sbroggio, M. et al. (2011). IQGAP1 regulates ERK1/2 and AKT signaling in the heart and sustains functional remodeling upon pressure overload. Cardiovasc Res doi: 10.1093/cvr/cvr103.Visavadiya, NP. et al. (2011). High glucose upregulates upstream stimulatory factor 2 in human renal proximal tubular cells through angiotensin II-dependent activation of CREB. Exp Nephro doi: 10.1159/000320593.Yoshikawa, A. et al. (2011). The (pro)renin receptor is cleaved by ADAM19 in the Golgi leading to its secretion into extracellular space. Hpertension 34, 599.Feliers, D. et al. (2010). Mechanism of VEGF expression by high glucose in proximal tubule epithelial cells. Mol and Cell Endocrinol 314, 136.Iwai, M. et al. (2010). Direct renin inhibition improved insulin resistance and adipose tissue dysfunction in type 2 diabetic KK-A y mice. J Hypertens 28, 1471.Schmiedt, CW. et al. (2010). Effects of renal autograft ischemic storage and reperfusion on intraoperative hemodynamic patterns and plasma renin concentrations in clinically normal cats undergoing renal autotransplantation and contralateral nephrectomy. Am J Vet Res 71, 1220.Du, D. et al. (2009). Expression of protein complex comprising the human prorenin and (pro)renin receptor in silkworm larvae using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmids for improving biological function. Mol Biotech 43, 1559.Schmiedt, C. et al. (2009). Measurement of plasma renin concentration in cats by use of a fluorescence resonance energy transfer peptide substrate of renin. American J Vet Res 70, 1315.ivná, M. et al. (2009).Dominant renin gene mutations associated with early-onset hyperuricemia, anemia, and chronic kidney failure. Am J Hum Genet 85, 204.Wei, Y. et al. (2009). Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases. Bioorganic and Medicinal Chem 17, 3003. |
