| Product Name | SensoLyte ® AMC tPA Activity Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72160 |
| US$ | $479 |
Tissue-type plasminogen activator (tPA) is a serine protease that cleaves pro-enzyme plasminogen into active plasmin. It has been recognized that tPA play important roles in various physiological and pathological processes.In plasma, it functions as a thrombolytic enzyme and can be used for treatment of ischemic stroke. In the central nervous system, tPA promotes events associated with synaptic plasticity and cell death in a number of settings, such as cerebral ischemia and seizures. The SensoLyte® AMC tPA Activity Assay Kit is optimized for assaying tPA enzyme activities. This kit contains a fluorogenic substrate with a high reactivity and low background.tPA cleaves the substrate resulting in release of AMC (7-amino-4-methylcoumarin) fluorophore.Fluorescence can be monitored at excitation /emission= 354/442 nm. This kit can be used for high throughput screening of tPA inducers and inhibitors. Kit size: 100 assays. | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Product Citations | Ambadapadi, S. et al. (2015) Reactive Center Loop (RCL) peptides derived from serpins display independent coagulation and immune modulating activities J. Biol. Chem. 291 2874-2887doi: 10.1074/jbc.M115.704841Rodier, M. et al. (2014). Exogenous t-PA administration increases hippocampal mature BDNF levels. Plasmin-or NMDA-dependent mechanism? PloS one 9, e92416. |
