| Product Name | SensoLyte ® AFC Plasmin Activity Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72124 |
| US$ | $380 |
Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis. The SensoLyte® AFC Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity. The assay utilizes a fluorogenic peptide, which upon enzyme cleavage releases the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. The fluorescent signal can be measured at Ex/ Em =380 nm/500 nm. This assay can detect picogram level of plasmin activity. Assays are performed in a convenient 96-well microplate format. Kit size: 100 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Product Citations | Ambadapadi, S. et al. (2015) Reactive Center Loop (RCL) peptides derived from serpins display independent coagulation and immune modulating activities J. Biol. Chem. 291 2874-2887doi: 10.1074/jbc.M115.704841Portelli, MA. et al. (2014). Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels. FASEB J 28, 923.Park, HJ. et al. (2013). Identification of a specific haptoglobin C‐terminal fragment in arthritic synovial fluid and its effect on interleukin‐6 expression. Immunol 140, 133. doi: 10.1111/imm.12125.Sasaki, T. et al. (2012). Intravenous and oral administrations of DD2 [7-Amino-2-(sulfanylmethyl)heptanoic acid] produce thrombolysis through inhibition of plasma TAFIa in rats with tissue factor-induced microthrombosis. Thrombosis Res 130, e222. doi:10.1038/jid.2012.204Stewart, CE. et al. (2012). uPAR regulates bronchial epithelial repair in vitro and is elevated in asthmatic epithelium. Thorax 67, 477.Tudpor, K. et al. (2012). Urinary plasmin inhibits TRPV5 in nephrotic-range proteinuria. J Am Soc Nephrol 23, 1824. |
