| Product Name | SensoLyte ® FDP Protein Phosphatase Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-71100 |
| US$ | $300 |
Protein phosphatases have received great attention as drug-screening targets. FDP is a highly sensitive fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The phosphatase-induced hydrolysis of FDP yields an intense fluorescent product that has excitation wavelength around 490 nm, and emission maximum around 520 nm. The fluorescence of FDP hydrolyzed product is strengthened when pH > 7.0. For some protein phosphatase requiring slightly acidic buffer for reaction, a pH-adjusting stop solution should be added before reading the fluorescence signal. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inducers and inhibitors.Kit Size: 500 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C |
| Product Citations | Zhang, Y. et al. (2016). Dopamine D2 receptors" effects on renal inflammation are mediated by regulation of PP2A function.AJP Renal Physio 310, 2, F128-134.https://doi.org/10.1152/ajprenal.00453.2014Bica, L. et al. (2014). Neuroprotective Copper Bis (thiosemicarbazonato) complexes promote Neurite Elongation. PloS one 9, e90070.Xie, ZZ. et al. (2014). Sulfhydration of p66Shc at cysteine59 mediates the anti-oxidant effect of Hydrogen Sulfide. Antioxidants Redox Signal doi:10.1089/ars.2013.5604.Savinov, A. et al. (2012). The Bxb1 gp47 recombination directionality factor is required not only for prophage excision, but also for phage DNA replication. Gene 495, 42.Zhang, X. et al. (2009). A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A. J. Cell Biol. 184, 67.Kirchberger, S. et al. (2008). The cytoplasmic tail of CD45 is released from activated phagocytes and can act as an inhibitory messenger for T cells. Blood 112, 1240. |
