| Product Name | SensoLyte ® 520 MMP - 8 Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-71154 |
| US$ | $480 |
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-8 (neutrophil collagenase) is proposed as a potential anti-cancer drug target. SensoLyte® 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities.In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format. Kit size:100 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C |
| Product Citations | Schubert-Unkmeir, A. et al. (2010). Neisseria meningitidis Induces Brain Microvascular Endothelial Cell Detachment from the Matrix and Cleavage of Occludin: A Role for MMP-8. PLoS Pathog 6, 4.Slanina, H. et al. (2010). Real-time impedance analysis of host cell response to meningococcal infection. J Microbiol Met 84, 101. |
