The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. A monoclonal anti-human-MMP-2 antibody is employed to pull down MMP-2 from the mixture first. MMP-2 activity is then quantified by a 5-FAM/QXL^® 520 fluorescence resonance energy transfer (FRET) peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-2-induced cleavage of the FRET substrate. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. Ample materials are provided to perform 96 assays in a 96-well format.

Figure 1. Specificity of SensoLyte® Plus 520 MMP-2 assay kit. APMA-activated MMPs, 30 ng each, are added to the anti-MMP-2 antibody pre-coated microplate. After incubation, the plate was washed and the activity of MMPs detected by 5-FAM/QXL^® 520 FRET peptide substrate. 18 hrs after adding the substrate, fluorescence signal was monitored at Ex/Em=490/520 nm (FlexStation 384II). The reading from all wells was subtracted with the reading from blank control, which contains FRET substrate but no MMPs. (n=3, mean±S.D.)