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当前位置: 首页 > 产品中心 > Transporters > Anaspec/SensoLyte®520颗粒酶A活性测定试剂盒,荧光法 NEW/1套件/AS-72260
商品详细Anaspec/SensoLyte®520颗粒酶A活性测定试剂盒,荧光法 NEW/1套件/AS-72260
Anaspec/SensoLyte®520颗粒酶A活性测定试剂盒,荧光法&nbspNEW/1套件/AS-72260
Anaspec/SensoLyte®520颗粒酶A活性测定试剂盒,荧光法 NEW/1套件/AS-72260
商品编号: AS-72260
品牌: Anaspec
市场价: ¥9600.00
美元价: 4800.00
产地: 美国(厂家直采)
公司:
产品分类: 转运体
公司分类: Transporters
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
Product NameSensoLyte® 520 Granzyme A Activity Assay Kit, FluorimetricNEW
Size1 kit
Catalog #AS-72260
US$$480

Granzyme A is the most abundant serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A enters pathogen-containing and tumor cells by the perforin pathway and induces caspase-independent apoptosis. Thus, cells that are resistant to the caspases or Granzyme B (a chief apoptotic mediator) might be sensitive to Granzyme A.Recent studies showed that Granzyme A activates a novel programmed cell death pathway that begins in the mitochondrion. Granzyme A also targets several important nuclear proteins for degradation including histones and DNA damage repair proteins. Granzyme A has proinflammatory activity inducing certain cytokines secretion. SensoLyte®520 Granzyme A Assay Kit provides a convenient assay for screening of enzyme inhibitors or for detecting the activity of enzyme using a novel internally quenched 5-FAM/QXL® FRET substrate. Upon cleavage by granzyme A, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. This kit can be used to detect the activity of Granzyme A enzyme in biological samples and purified enzyme preparations.The kit contains:

  • QXL® 520/ 5-FAM-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage)
  • Assay buffer
  • Inhibitor
  • Fluorescence reference standard for calibration
  • A detailed protocol
Kit size: 100 assays (96-well plate)
Detailed InformationDatasheet
Material Safety Data Sheets (MSDS)
Storage-20°C
品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中,包括真菌,昆虫,两栖动物和人类中,已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素,杀真菌,溶血,杀病毒和杀肿瘤活性。基于它们的结构特性,这些抗菌肽可分为三类:螺旋状肽,含有一个至几个二硫键的肽以及富含某些氨基酸(例如脯氨酸或色氨酸)的肽。这些肽大多数具有一些共同的特征,例如它们的低分子量(2-5 kDa),存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制,但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素,其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A,B和D是紧密的同源物,由天蚕素蛾的35中发现的35-39个残基组成。