| Product Name | SensoLyte ® Green Protease Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-71124 |
| US$ | $286 |
The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate"s fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuouslymeasure the kinetics of a variety of exopeptidases and endopeptidases. The kit contains: Fluorescent labeled casein with high ratio of dye/protein (pH-insensitive) Trypsin (as positive control) Assay buffer A detailed protocol Kit Size: 500 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C |
| Product Citations | Teshigahara Y, et al. (2020) A Chondroitin Sulfate Chain of Urinary Trypsin Inhibitor Enhances Protease Inhibitory Activity of the Core Protein. Journal of Applied Glycoscience. jag-JAG. doi: 10.5458/jag.jag.JAG-2019_0021Kao, W-T. et al. (2012). Antioxidant and trypsin inhibition activity of dioscorin influenced by yam cultivars and eluent pH. Intl J Food Sci Tech 47, 1384. doi: 10.1111/j.1365-2621.2012.02984.xWei, Y. et al. (2009). Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases. Bioorganic and Medicinal Chem 17, 3003.Groll, M. et al. (2008). A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanism. Nature 452, 755.Phuong, D. et al. (2008). Inhibitory effects of antrodins AE from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. Phytother Res 23, 582. |
