| Product Name | SensoLyte ® Rh110 Factor Xa Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72207 |
| US$ | $480 |
Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits. FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca2+ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases. The SensoLyte® Rh110 Factor Xa Assay Kit provides a convenient assay for screening of FXa inhibitors or continuous assay of enzyme activity using a fluorogenic substrate.Upon FXa protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore which has a bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. Kit size: 100 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Product Citations | Lagos, F. C. et al. (2017). Novel FXa Inhibitor Identification through Integration of Ligand- and Structure-Based Approaches molecules 22 1588, doi:10.3390/molecules22101588 |
