| Product Name | Sensolyte ® Cell Viability and Proliferation Assay Kit- Larger Size |
| Size | 1 kit |
| Catalog # | AS-71301 |
| US$ | $631 |
Lactate dehydrogenase (LDH) exists ubiquitously in the cytoplasm of living mammalian cells and the measurement of cytoplasmic LDH activity is a well-accepted assay to quantify living cell numbers and indicate cell viablity. The SensoLyte® Cell Viability and Proliferation Assay Kit provides researchers with a convenient one-solution and one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. In this kit, resazurin is used as a sensitive fluorogenic indicator. Resazurin is converted to the strongly fluorescent resorufin (Ex/Em=560nm/590 nm) by cytoplasmic LDH. The kit can detect as few as 48 living cells with a linear range up to 5X104 cells (R2=0.99). This kit can also be used for high throughput screening of cell proliferation or cytotoxicity effect of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. Kit Size: 10,000 assays This assay is also available in a smaller size (2000 assays) - Cat# 71300. | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C |
| Product Citations | Karjalainen, K. et al. (2011). Targeting neuropilin-1 in human leukemia and lymphoma. Blood 117, 920.Walsh, LA. et al. (2011). IGF-1 increases invasive potential of MCF 7 breast cancer cells and induces activation of latent TGF-β1 resulting in epithelial to mesenchymal transition. Cell Commun Signaling 10.1186/1478-811X-9-10.Brennan, SE. et al. (2009). The mRNA-destabilizing protein tristetraprolin is suppressed in many cancers, altering tumorigenic phenotypes and patient prognosis. Cancer Res 69, 5168.Roelants, V. et al. (2008). Comparison between adenoviral and retroviral vectors for the transduction of the thymidine kinase PET reporter gene in rat mesenchymal stem cells. J Nucl Med 49, 1836.Siegfried, JM.et al. (2007). Signaling pathways involved in cyclooxygenase-2 induction by hepatocyte growth factor in non-small cell lung cancer. Mol Pharmacol 10.1124/mol.107.034215. |
