4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:原厂直采,平行进口,授权代理(蚂蚁淘为您服务)
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Stimulus > Anaspec/SensoLyte和174;AMC钙蛋白酶活性测定试剂盒*荧光法*/1试剂盒/AS-72150
商品详细Anaspec/SensoLyte和174;AMC钙蛋白酶活性测定试剂盒*荧光法*/1试剂盒/AS-72150
Anaspec/SensoLyte和174;AMC钙蛋白酶活性测定试剂盒*荧光法*/1试剂盒/AS-72150
Anaspec/SensoLyte和174;AMC钙蛋白酶活性测定试剂盒*荧光法*/1试剂盒/AS-72150
商品编号: AS-72150
品牌: Anaspec
市场价: ¥10440.00
美元价: 5220.00
产地: 美国(厂家直采)
公司:
产品分类: 刺激/抑制试剂
公司分类: Stimulus
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
Product NameSensoLyte ® AMC Calpain Activity Assay Kit *Fluorimetric*
Size1 kit
Catalog #AS-72150
US$$522

The calpains are a family of intracellular Ca2+ supdependent cysteine proteases. The best-characterized calpains are isoforms 1 (µ) and 2 (m). Calpains respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). They are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena, such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer"s disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.The SensoLyte® AMC Calpain Assay Kit is optimized for detecting calpain activities. This kit contains a fluorogenic peptide substrate, suc-LLVY-AMC. Upon calpain protease cleavage, suc-LLVY-AMC generates the AMC fluorophore emitting bright blue fluorescence and can be monitored at excitation/emission=354/442 nm. Increase in AMC fluorescence is proportional to calpain activity. The assay can detect both calpain 1 (μ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The assays are performed in a convenient 96-well microplate format.Kit size: 100 assaysRELATED PRODUCT:SensoLyte® 520 Calpain Activity Assay Kit (Ex/Em=490/520 nm)

Detailed InformationDatasheet
Material Safety Data Sheets (MSDS)
Poster
Product CitationsCotten, SW. et al. (2014). Genetic myostatin decrease in the golden retriever muscular dystrophy model does not significantly affect the ubiquitin proteasome system despite enhancing the severity of disease. Am J Transl Res 6, 43.Lai, YS. et al. (2011). Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli. Front Microbiol 2, 222.doi:10.3389/fmicb.2011.00222.Wadosky, KM. et al. (2009). Regulation of the calpain and ubiquitin proteasome systems in a canine model of muscular dystrophy. Muscle & Nerve DOI: 10.1002/mus.22125.
品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中,包括真菌,昆虫,两栖动物和人类中,已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素,杀真菌,溶血,杀病毒和杀肿瘤活性。基于它们的结构特性,这些抗菌肽可分为三类:螺旋状肽,含有一个至几个二硫键的肽以及富含某些氨基酸(例如脯氨酸或色氨酸)的肽。这些肽大多数具有一些共同的特征,例如它们的低分子量(2-5 kDa),存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制,但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素,其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A,B和D是紧密的同源物,由天蚕素蛾的35中发现的35-39个残基组成。