商品详细Anaspec/SensoLyte®蓝色葡萄糖脑苷酶（GBA）分析试剂盒*荧光法*
Anaspec/SensoLyte®蓝色葡萄糖脑苷酶（GBA）分析试剂盒*荧光法*
市场价：
¥9600.00
美元价：
4800.00
商品介绍
| Product Name | SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*NEW |
| Size | 1 kit |
| Catalog # | AS-72258 |
| US$ | $480 |
DescriptionGlucocerebrosidase/GBA (also called acid β-glucosidase, Glucosylceramidase,) is a lysosomal enzyme responsible for the breakdown of glucocerebroside releasing glucose and ceramide. Deficiency of this enzyme due to genetic mutations leads to accumulation of glucocerebroside and development of lysosomal storage disease, known as Gaucher disease (GD). Mutations in the glucocerebrosidase (GBA1) gene are also associated with increased risk for Parkinson disease and related disorders. It has been hypothesized that GBA, when not available to clear out proteins like alpha-synuclein, results in the accumulation of the proteins thereby contributing to Parkinsons disease. The SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a most common fluorogenic substrate, MUGlc, for measurement of GBA activity. In presence of GBA the colorless substrate is hydrolyzed into 4-methylumbelliferone (4MU), and after addition of stop solution a bright blue fluorescence can be monitored at excitation/emission = 365nm/445nm nm. Related Product (ex/em=570nm/610nm)SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*Fig 1.Inhibition of GBA activity by Isofagomine as measured withSensoLyte®Blue Glucocerebrosidase Assay Kit.Fig 2.Activation of GBAactivity by GCase Activator(NCGC00188758). 5ng of GBA enzyme was incubated with and without 10µM of GCaseactivator and fluorescence was measured withSensoLyte®Blue Glucocerebrosidase Assay Kit. |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | Component C at -80 °C, Component A, B, D, E at -20 °C and Component A and B avoid light and moisture |
| References | - Xu, Y. H et al, Am.J.Pathol, 163, 2093(2003)
- Sidransky, E et al, N Engl J Med, 361, 1651(2009)
- Murphy K.E et al, Brain,137,834(2014)
- Sybertz, E. et al, J Lipid Res 55, 1996 (2014)
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品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中，包括真菌，昆虫，两栖动物和人类中，已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素，杀真菌，溶血，杀病毒和杀肿瘤活性。基于它们的结构特性，这些抗菌肽可分为三类：螺旋状肽，含有一个至几个二硫键的肽以及富含某些氨基酸（例如脯氨酸或色氨酸）的肽。这些肽大多数具有一些共同的特征，例如它们的低分子量（2-5 kDa），存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制，但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素，其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A，B和D是紧密的同源物，由天蚕素蛾的35中发现的35-39个残基组成。
