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当前位置: 首页 > 产品中心 > Metal_bath > Anaspec/SensoLyte和174;红色谷氨酰胺转胺酶检测试剂盒,荧光法/1试剂盒/AS-72245
商品详细Anaspec/SensoLyte和174;红色谷氨酰胺转胺酶检测试剂盒,荧光法/1试剂盒/AS-72245
Anaspec/SensoLyte和174;红色谷氨酰胺转胺酶检测试剂盒,荧光法/1试剂盒/AS-72245
Anaspec/SensoLyte和174;红色谷氨酰胺转胺酶检测试剂盒,荧光法/1试剂盒/AS-72245
商品编号: AS-72245
品牌: Anaspec
市场价: ¥9600.00
美元价: 4800.00
产地: 美国(厂家直采)
公司:
产品分类: 金属浴
公司分类: Metal_bath
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
Product NameSensoLyte ® Red Transglutaminase Assay Kit, Fluorometric
Size1 kit
Catalog #AS-72245
US$$480

Transglutaminases (TGs) are Ca2+and thiol-dependent enzymes that catalyze posttranslational modifications ofproteins. These covalent reactions include formation of isopeptide linkagesbetween the carboxamide groups of protein-bound glutamine residues and ε-aminogroups of protein-bound lysine and polyamine residues, hydrolysis and esterification of glutamine residues. Family of TGs includes atleast nine members: TG 1-7, coagulation factor XIIIa, and the keratinocytemembrane bound TG form. TG hasattracted considerable interest as a potential drugtarget. Its activity is thought to contribute to Alzheimer disease,Parkinson disease, Huntington disease, and supranuclear palsy.

SensoLyte®Transglutaminase Activity Assay Kit provides a convenient assay for the highthroughput screening of TG modulators and inhibitors. TG catalyzes covalentbond formation between a free amine group of poly-D-lysine, which is coated onthe plate surface, and γ-carboxamide group of glutamine in biotin-TVQQELpeptide substrate. The reaction immobilizes biotin-conjugated peptide to theplate surface. The amount of immobilized biotin is determined usingstreptavidin-horseradishperoxidase (HRP), 10-Acetyl-3,7-dihydroxyphenoxazine(ADHP), and hydrogen peroxide (H2O2).The non-fluorescent ADHP is oxidized to the strong fluorescent resorufin in thepresence of H2O2 by peroxidases, such as HRP. The fluorescence intensity of resorufin isproportional to the activity of TG in a sample. Assay is performed in aconvenient 96-well microplate format.

Detailed InformationDatasheet
Material Safety Data Sheets (MSDS)
品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中,包括真菌,昆虫,两栖动物和人类中,已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素,杀真菌,溶血,杀病毒和杀肿瘤活性。基于它们的结构特性,这些抗菌肽可分为三类:螺旋状肽,含有一个至几个二硫键的肽以及富含某些氨基酸(例如脯氨酸或色氨酸)的肽。这些肽大多数具有一些共同的特征,例如它们的低分子量(2-5 kDa),存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制,但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素,其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A,B和D是紧密的同源物,由天蚕素蛾的35中发现的35-39个残基组成。