Anaspec/SensoLyte和174；520 TACE（α；-分泌酶活性测定试剂盒*荧光法*/1试剂盒/AS-72085
市场价：
¥9600.00
美元价：
4800.00
产品分类：
真空泵
公司分类：
Vacuum_pump
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Product Name | SensoLyte ® 520 TACE (α - Secretase) Activity Assay Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-72085 |
| US$ | $480 |
TACE (tumor necrosis factor-alpha converting enzyme), α-secretase, or ADAM17 is a member of the ADAM family of proteases that contain both disintegrin and metalloprotease (catalytic) domains. This enzyme is involved in sperm-egg binding and fusion, muscle cell fusion, neurogenesis, modulation of Notch receptor and ligand processing, and processing of the pro-inflammatory cytokine, TNFα. TACE is currently being explored as a target for anti-inflammatory drugs. SensoLyte® 520 TACE Activity Assay Kit uses a 5-FAM (fluorophore) and QXL® 520 (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by the active enzyme, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL® 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The kit can be used to detect the activity of TACE or screen for TACE inhibitors. The assays are performed in a convenient 96-well format. Kit size: 100 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) Poster |
| Storage | -20°C |
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J Biol Chem 288, 12448. doi: 10.1074/jbc.M113.454405.Tripathy, D. et al. (2013). Pioglitazone improves glucose metabolism and modulates skeletal muscle TIMP-3TACE dyad in type 2 diabetes mellitus: a randomised, double-blind, placebo-controlled, mechanistic study. Diabetologia 56, 2153. doi: 10.1007/s00125-013-2976-z.Wang, Y. et al. (2013). Strain-induced differentiation of fetal type II epithelial cells is mediated via the Integrin α6β1-ADAM17/Tumor Necrosis Factor-α-converting Enzyme (TACE) Signaling Pathway. J Biol Chem 288, 25646. doi: 10.1074/jbc.M113.473777.Akool, ES. et al. (2012). Cyclosporin A and tacrolimus induce renal Erk1/2 pathway via ROS-induced and metalloproteinase-dependent EGF-receptor signaling. Biochem Pharmacol 83, 286. doi: 10.1016/j.bcp.2011.11.001.Catalán, U. et al. (2012). Inhibition of the transcription factor c-Jun by the MAPK family, and not the NF-kB pathway, suggests that peanut extract has anti-inflammatory properties. Mol Immunol 52, 125. doi: 10.1016/j.molimm.2012.05.007.Chanthaphavong, RS. et al. (2012). A Role for cGMP in inducible nitric-oxide synthase (iNOS)-induced Tumor Necrosis Factor (TNF) α-converting Enzyme (TACE/ADAM17) activation, translocation, and TNF Receptor 1 (TNFR1) shedding in hepatocytes. J Biol Chem 287, 35887. doi: 10.1074/jbc.M112.365171.Costa, GN. et al. (2012). Contribution of TNF receptor 1 to retinal neural cell death induced by elevated glucose. Mol Cell Neurosci 50, 113. doi: 10.1016/j.mcn.2012.04.003.Jijon, HB. et al. (2012). The EGF receptor and HER2 participate in TNF-α-dependent MAPK activation and IL-8 secretion in intestinal epithelial cells. Mediat Inflamm 2012, 12 pages. doi: 10.1155/2012/207398Marolda, R. et al. (2012). Substance P activates ADAM9 mRNA expression and induces α-secretase-mediated amyloid precursor protein cleavage. Neuropharmacol 62, 1954. doi: 10.1016/j.neuropharm.2011.Schmidt, V. et al. (2012). Quantitative modelling of amyloidogenic processing and its influence by SORLA in Alzheimers disease. EMBO 31,187. doi: 10.1038/emboj.2011.352.Cardellini, M. et al. (2011). Decreased IRS2 and TIMP3 expression in monocytes from offspring of Type 2 diabetic patients is correlated with insulin resistance and increased Intima-Media thickness. Diabetes 60, 3265. doi: 10.2337/db11-0162.Guma, M. et al. (2011). Constitutive intestinal NF-kB does not trigger destructive inflammation unless accompanied by MAPK activation. J Exp Med 208, 1889. doi: 10.1084/jem.20110242.Oguma, T. et al. (2011). Induction of Mucin and MUC5AC expression by the protease activity of Aspergillus fumigatus in airway epithelial cells. J Immunol 187, 999. doi: 10.4049/jimmunol.1002257.Roher, N. et al. (2011). Endotoxin recognition in fish results in inflammatory cytokine secretion not gene expression. Innate Immunity 17, 16. doi: 10.1177/1753425909348232.Stöhr, O. et al. (2011). Insulin receptor signaling mediates APP processing and β-amyloid accumulation without altering survival in a transgenic mouse model of Alzheimer"s disease. Age 35, 83. doi: 10.1007/s11357-011-9333-2.Takasugi, N. et al. (2011). BACE1 activity is modulated by cell-associated Sphingosine-1-Phosphate. J Neurosci 31, 6850. doi: 10.1523/JNEUROSCI.6467-10.2011.Yang, Y. et al. (2011). Tissue inhibitor of metalloproteinases-3 mediates the death of immature oligodendrocytes via TNF-α/TACE in focal cerebral ischemia in mice. J Neuroinflam 8, 108. doi: 10.1186/1742-2094-8-108.Zhao, XQ. et al. (2011). CRP enhances soluble LOX-1 release from macrophages by activating TNF-α converting enzyme. J Lipid Res doi: 10.1194/jlr.M015156.Hassemer, EL. et al. (2010). The waved with open eyelids (woe) locus is a hypomorphic mouse mutation in Adam17. Genetics 185, 245. doi: 10.1534/genetics.109.113167.Cardellini, M. et al. (2009). TIMP3 is reduced in atherosclerotic plaques from subjects with type 2 diabetes and increased by SirT1. Diabetes 58, 2396. doi: 10.2337/db09-0280.Fiorentino, L. et al. (2009). Increased tumor necrosis factor α-converting enzyme activity induces insulin resistance and hepatosteatosis in mice. Hepatol 51, 103. doi: 10.1002/hep.23250.Hurst, LA. et al. (2009). Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment. J Neuroimmunol 210, 108. doi: 10.1016/j.jneuroim.2009.02.008.Walker, EJ. et al. (2009). TIMP-3 and MMP-3 contribute to delayed inflammation and hippocampal neuronal death following global ischemia. Exp Neuro 216, 122. |
品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中，包括真菌，昆虫，两栖动物和人类中，已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素，杀真菌，溶血，杀病毒和杀肿瘤活性。基于它们的结构特性，这些抗菌肽可分为三类：螺旋状肽，含有一个至几个二硫键的肽以及富含某些氨基酸（例如脯氨酸或色氨酸）的肽。这些肽大多数具有一些共同的特征，例如它们的低分子量（2-5 kDa），存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制，但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素，其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A，B和D是紧密的同源物，由天蚕素蛾的35中发现的35-39个残基组成。
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