Neprilysin (NEP) is a transmembrane metallopeptidase normally expressed by a variety of tissues. It is also known as neutral endopeptidase, and enkephalinase.NEP cleaves peptides at the N-terminal side of hydrophobic amino acid residues and is responsible for the degradation and inactivation of a variety of physiological substrates. NEP is a major extracellular amyloid beta-peptide degrading enzyme in the brain and targeting NEP is considered a potential therapeutic strategy for the prevention and treatment of Alzheimer"s disease. NEP has also been implicated in the pathogenesis of hypertension, diabetes, and cancer. The SensoLyte® 520 Neprilysin Assay Kit employs a novel internally quenched 5-FAM/QXL® FRET substrate for the detection of neprilysin activity.The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 0.78 ng/mL of active Neprilysin.

The Kit contains:

• 5-FAM/QXL^® 520 Neprilysin substrate, Ex/Em=490/520 nm upon cleavage • 5-FAM• Fluorescence reference standard, Ex/Em=490/520 nm• Recombinant human Neprilysin• 2X Assay BufferInhibitor