| Product Name | SensoLyte ® pNPP Protein Phosphatase Assay Kit *Colorimetric* |
| Size | 1 kit |
| Catalog # | AS-71105 |
| US$ | $268 |
Protein phosphatases have received great attentions as drug-screening targets. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for protein tyrosine phosphatases and serine/threonine phosphatases. SensoLyte® pNPP Protein Phosphatase Assay Kit uses pNNP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. It has high sensitivity and wide linear range (The detection limit is generally 3 ng or below). The kit contains: pNNP chromogenic substrate Protein phosphatase inhibitor Assay buffer A "mix and read" assay protocol that is compatible with HTS liquid handling instruments Kit size: 500 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C |
| References | Ref: Urbanek RA et al (2001) Potent reversible inhibitors of the protein tyrosine phosphatase CD45. J Med Chem. 44, 1777-93; Marley AE et al (1996) Biochemical characterization and deletion analysis of recombinant human protein phosphatase 2C alpha. Biochem J. 320, 801-6. |
| Molecular Weight | N/A |
| Product Citations | Linford, AS. et al. (2014). Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase. Mol Biochem Parasitol 193, 33.Rebollo, A. et al. (2014). Liquid fructose downregulates Sirt1 expression and activity and impairs the oxidation of fatty acids in rat and human liver cells. Biochim Biophys Acta (BBA)-Mol Cell Biol Lipids 1841, 514.Okon, IS. et al. (2014). Liver kinase B1 expression promotes phosphatase activity and abrogation of receptor tyrosine kinase phosphorylation in human cancer cells. J Biol Chem 289, 1639.Uhrig, R. et al. (2011). Two ancient bacterial-like PPP family phosphatases from arabidopsis are highly conserved plant proteins that possess unique properties. Plant Physiology 157, 1778-1792.Lin, C. et al. (2010). Impaired dephosphorylation renders G6PD-knockdown HepG2 cells more susceptible to H2O2-induced apoptosis. Free Radical Biol Med 49, 361.Svensson, M. et al. (2009). Heat Stabilization of the Tissue Proteome: A New Technology for Improved Proteomics. J Proteome Res 8, 974.Cho, Y. et al. (2008). Tyrosine phosphatase SHP-1 in oxidative stress and development of allergic airway inflammation. Am J Respir Cell Mol Biol 39, 412. doi: 10.1165/rcmb.2007-0229OCCoyne, CB. et al. (2007) Poliovirus entry into human brain microvascular cells requires receptor-induced activation of SHP-2. EMBO J 26, 4016. doi: 10.1038/sj.emboj.7601831 |
