| Product Name | SensoLyte ® MG Phosphate Assay Kit *Colorimetric* |
| Size | 1 kit |
| Catalog # | AS-71103 |
| US$ | $300 |
The MG (Malachite Green) Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate, and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes, or multi-well plates. The assays can be conveniently performed in 96 or 384-well plates for high-throughput screening of enzyme inhibitors. The kit has been used for assaying phosphatase, phospholipase or lipase, nucleoside triphosphatase (ATPase, GTPase, etc.) and phytase. The kit has the followingcomponents: Highly purified Malachite Green reagent Protein phosphatase inhibitor Assay buffer A "mix and read" assay protocol that is compatible with HTS liquid handling instruments with Z" factors of 0.7 to 0.9. Kit size: 1000 assays | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | Room temperature |
| References | Ref: Gurpal S. et al. (2003) Characterization of organic phosphorus in leachate from a grassland soil. Soil Biol.ogy & Biochemistry 35, 1317-1323; Cogan EB (1999) A robotics-based automated assay for inorganic and organic phosphates. Anal Biochem. 271, 29-35; Fisher DK and Higgins TJ (1994) A sensitive, high-volume, colorimetric assay for protein phosphatases. Pharm Res.11, 759-63; Henkel RD (1988) A microassay for ATPase. Anal Biochem. 169, 312-318. |
| Molecular Weight | N/A |
| Product Citations | Hwang, IW. et al. (2014). Human acetyl-CoA carboxylase 2 expressed in silkworm Bombyx mori exhibits posttranslational biotinylation and phosphorylation. Appl Microbiol Biotechnol 1.Lahiri, T. et al. (2014). A structural basis for the regulation of an H-NOX-associated cyclic-di-GMP synthase/phosphodiesterase enzyme by nitric oxide-bound H-NOX. Biochem 53, 2126.Maione, F. et al. (2014). Interleukin-17A exacerbates ferric chloride-induced arterial thrombosis in rat carotid artery. Intl J Inflamm, 2014. doi: http://dx.doi.org/10.1155/2014/247503.Day, S. et al. (2013). Impaired assembly and post-translational regulation of 26S proteasome in human end-stage heart failure. Circ Heart Fail 6, 544. doi: 10.1161/CIRCHEARTFAILURE.112.000119.Lu, D & PA. Insel (2013). Hydrolysis of extracellular ATP by ectonucleoside triphosphate diphosphohydrolase (ENTPD) establishes the set point for fibrotic activity of cardiac fibroblasts. J Biol Chem 288, 19040Midde, K. et al. (2013). Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain. J Biol Chem 288, 7012. doi: 10.1074/jbc.M112.434209.Zaparaty, LM. et al. (2013). The first prokaryotic trehalose synthase complex identified in the hyperthermophilic Crenarchaeon Thermoproteus tenax. PlosOne. doi: 10.1371/journal.pone.0061354.Manque, PA. et al. (2012). Identification and characterization of a novel calcium-activated apyrase from Cryptosporidium parasites and its potential role in pathogenesis. PLoS 7, e31030. doi:10.1371/journal.pone.0031030Repen, B. et al. (2012). Optimization of a malachite green assay for detection of ATP hydrolysis by solubilized membrane proteins. Anal Biochem http://dx.doi.org/10.1016/j.ab.2012.04.016Clayton, A. et al. (2011). Cancer Exosomes Express CD39 and CD73, Which Suppress T Cells through Adenosine Production. J Immunol 187, 676. doi: 10.4049/jimmunol.1003884.Hilaire, C. et al. (2011). NT5E mutations and arterial calcifications. N Engl J Med 364, 432.Oomah, BD. et al. (2011). Phenolics, phytic acid, and phytase in Canadian-grown low-tannin Faba Bean (Vicia faba L.) genotypes. J Agric Food Chem 10.1021/jf200338b.Pinust, F. et al. (2011). Purification, primary structure, and properties of Euphorbia characias latex purple acid phosphatase. Biochem 76, 694.Yang, Z. et al. (2011). Characterization of LipL as a non-heme, Fe(II)-dependent α-ketoglutarate:UMP dioxygenase that generates uridine-5"-aldehyde during A-90289 biosynthesis. J Biol Chem 10.1074/jbc.M110.203562.Vickers, K. et al. (2010). Lyso-phosphatidylcholine induces osteogenic gene expression and phenotype in vascular smooth muscle cells. Atherosclerosis 211, 122.Wolf, A. et al. (2010). Plakophilin 1 stimulates translation by promoting eIF4A1 activity. JCB 188, 463. |
