Anaspec/Calcein，AM*超纯级*，5 mM无水DMSO/200µL/AS-89203溶液
市场价：
¥3720.00
美元价：
1860.00
产品分类：
储存液
公司分类：
Storage_reagent
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
| Product Name | Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO |
| CAS | 148504-34-1 |
| Size | 200 µL |
| Catalog # | AS-89203 |
| US$ | $186 |
| Purity | ≥95% by HPLC |
Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability.In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.Hela cells stained with AnaSpecs Calcein AM fluorescent dye (Cat# AS-89201, AS-89202, AS-89203 and AS-89204). MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# AS-89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.
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| Images |  |
| Detailed Information |  Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| Molecular Weight | 994.857 |
| Molecular Formula | C46H46N2O23 |
| Spectral Properties | Abs/Em = 494/517 nm |
| Solvent System | DMSO |
| Product Citations | Robinson, M. et al. (2011). Screening of hepatitis C virus inhibitors using genotype 1a HCV replicon cell lines. Curr Prot Microbiol doi: 10.1002/9780471729259.mc1707s22.Delaunay, JL. et al. (2009). A missense mutation in ABCB4 gene involved in progressive familial intrahepatic cholestasis type 3 leads to a folding defect that can be rescued by low temperature. Hepatology 49, 1218.Zhang, K-H. et al. (2009). Ferritin heavy chainmediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 10.1158/0008-5472.CAN-09-0112. |
品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中，包括真菌，昆虫，两栖动物和人类中，已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素，杀真菌，溶血，杀病毒和杀肿瘤活性。基于它们的结构特性，这些抗菌肽可分为三类：螺旋状肽，含有一个至几个二硫键的肽以及富含某些氨基酸（例如脯氨酸或色氨酸）的肽。这些肽大多数具有一些共同的特征，例如它们的低分子量（2-5 kDa），存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制，但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素，其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A，B和D是紧密的同源物，由天蚕素蛾的35中发现的35-39个残基组成。
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