Anaspec/Calcein, AM/1 mg/AS-89201
市场价：
¥2860.00
美元价：
1430.00
产品分类：
缓冲液
公司分类：
buffer_solution
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Product Name | Calcein, AM |
| CAS | 148504-34-1 |
| Size | 1 mg |
| Catalog # | AS-89201 |
| US$ | $143 |
| Purity | ≥95% by HPLC |
Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability.In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.Hela cells stained with AnaSpecs Calcein AM fluorescent dye (Cat# AS-89201, AS-89202, AS-89203 and AS-89204). MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# AS-89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.
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| Images |  |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| Molecular Weight | 994.857 |
| Molecular Formula | C46H46N2O23 |
| Spectral Properties | Abs/Em = 494/517 nm |
| Solvent System | DMSO |
| Product Citations | Graham, DM. et al. (2012). Spatial manipulation of cells and organelles using single electrode dielectrophoresis. Int J of Life Sci Meth 52 , 39-43Kim, H. et al. (2011). Human macrophage ATP7A is localized in the trans-golgi apparatus, controls intracellular copper levels, and mediates macrophage responses to dermal wounds. Inflammation 10.1007/s10753-011-9302-z.Decaris, ML. et al. (2010). Design of experiments approach to engineer cell-secreted matrices for directing osteogenic differentiation. Annals Biomed Eng 39, 1174.Hernlem, B. et al. (2010). Dual fluorochrome flow cytometric assessment of yeast viability. Current Microbiol 61, 57.Miura, Y. et al. (2010). Change of morphological and functional characteristics of retinal pigment epithelium cells during cultivation of retinal pigment epithelium-choroid perfusion tissue culture. Opthalmic Res 43, 122.Zaveri, T. et al. (2010). Contributions of surface topography and cytotoxicity to the macrophage response to zinc oxide nanorods. Biomater 31, 2999.Decaris, ML. et al. (2009). Influence of the oxygen microenvironment on the proangiogenic potential of human endothelial colony forming cells. Angiogenesis 12, 303.Delaunay, JL. et al. (2009). A missense mutation in ABCB4 gene involved in progressive familial intrahepatic cholestasis type 3 leads to a folding defect that can be rescued by low temperature. Hepatology 49, 1218. Zhang, K-H. et al. (2009). Ferritin heavy chainmediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 10.1158/0008-5472.CAN-09-0112. |
品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中，包括真菌，昆虫，两栖动物和人类中，已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素，杀真菌，溶血，杀病毒和杀肿瘤活性。基于它们的结构特性，这些抗菌肽可分为三类：螺旋状肽，含有一个至几个二硫键的肽以及富含某些氨基酸（例如脯氨酸或色氨酸）的肽。这些肽大多数具有一些共同的特征，例如它们的低分子量（2-5 kDa），存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制，但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素，其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A，B和D是紧密的同源物，由天蚕素蛾的35中发现的35-39个残基组成。
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