Anaspec/Resorufinβ;-D-半乳吡喃糖苷/25 mg/AS-85617
市场价:
¥1960.00
美元价:
980.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Product Name | Resorufin β - D - Galactopyranoside |
Size | 25 mg |
Catalog # | AS-85617 |
US$ | $98 |
Purity | Minimum Purity: 95% |
Unlike FDG that requires a two-step hydrolysis to generate maximum fluorescence, resorufin β-D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence. This substrate is especially useful for sensitive enzyme measurements in ELISAs. The relatively low pKa (~6.0) of resorufin (the enzymatic hydrolysis product of resorufin galactoside) with Ex/Em=573/585 nm permits continuous measurement of enzymatic activity. Resorufin galactoside has also been used to quantitate β-galactosidase activity in single yeast cells by flow cytometry and to detect immobilized β-galactosidase activity. | |
Detailed Information | Material Safety Data Sheets (MSDS) |
Storage | -20°C desiccated and protected from light |
References | 1. Yang NC and Hu ML (2004).Anal Biochem 325, 337-43. 2. Chiocconi A, et al. (2000).Carbohydr Res 323, 7-13. 3. Hakamata W, et al. (2000).Carbohydr Res 324, 107-15.4. Weiss DJ, et al. (1997).Hum Gene Ther 8, 1545-54.5. Lukomskaya IS, et al. (1996).Clin Chim Acta 244, 145-54.6. Usui T, et al. (1992).Anal Biochem 202, 61-7.7. Cousin MA, et al. (1989).J Appl Bacteriol 66, 311-7.8. Bowen DM and Radin NS (1969). JNC 16, 457-460. |
Molecular Weight | 375.33 |
Molecular Formula | C18H17NO8 |
Spectral Properties | Abs/Em = 573/585 nm |
Solvent System | DMSO |
品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中,包括真菌,昆虫,两栖动物和人类中,已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素,杀真菌,溶血,杀病毒和杀肿瘤活性。基于它们的结构特性,这些抗菌肽可分为三类:螺旋状肽,含有一个至几个二硫键的肽以及富含某些氨基酸(例如脯氨酸或色氨酸)的肽。这些肽大多数具有一些共同的特征,例如它们的低分子量(2-5 kDa),存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制,但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素,其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A,B和D是紧密的同源物,由天蚕素蛾的35中发现的35-39个残基组成。
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