Anaspec/SensoLyte和174；均质AMC半胱天冬酶-3/7分析试剂盒/1试剂盒/AS-71118
市场价：
¥6300.00
美元价：
3150.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Product Name | SensoLyte ® Homogeneous AMC Caspase - 3/7 Assay Kit500 assays (96 - well plate) |
| Size | 1 kit |
| Catalog # | AS-71118 |
| US$ | $315 |
Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).The SensoLyte® Homogeneous AMC Capase-3/7 Assay Kit uses Ac-DEVD-AMC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AMC generates the AMC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=354 nm/442 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors. | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) Poster |
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Expression and function of survivin in canine osteosarcoma. Cancer Res 72, 249. doi:10.1158/0008-5472.CAN-11-2315Ansari, KI. et al. (2011). Fe(III)-salen and salphen complexes induce caspase activation and apoptosis in human cells. J Biomol Screen 16, 26.Fossey, S. et al. (2011). The novel curcumin analog FLLL32 decreases STAT3 DNA binding activity and expression, and induces apoptosis in osteosarcoma cell lines. BMC Cancer 11, 112.Gorski, JP. et al. (2011). Inhibition of proprotein convertase SKI-1 blocks transcription of key extracellular matrix genes regulating osteoblastic mineralization. J Biol Chem 286, 1836.Lin, TY. et al. (2011). AR-42, a novel HDAC inhibitor, exhibits biologic activity against malignant mast cell lines via downregulation of constitutively activated Kit. Blood 10.1182/blood-2009-07-231985.Rajaiah, R. et al. (2011). Interleukin-27 and Interferon-γ Are Involved in Regulation of Autoimmune Arthritis. J Biol Chem 286, 2817.Shin, J. et al. (2011). Herbal formula CGX ameliorates LPS/d-galactosamine-induced hepatitis. Food Chem Toxicol 10.1016/j.fct.2011.03.015.Yu, J. et al. (2011). Identification of components of grape powder with anti-apoptotic effects. Toxicol Ind Health 27, 19.Chen, L. et al. (2010). Hydrogen peroxide inhibits mTOR signaling by activation of AMPKα leading to apoptosis of neuronal cells. Lab Invest 90, 762.Lin, TY. et al. (2010). Characterization and modulation of canine mast cell derived eicosanoids. Vet Immunol Immunopathol 135, 118.Madhusoodhanan, R. et al. (2010). Effect of black raspberry extract in inhibiting NFκ B dependent radioprotection in human breast cancer cells. Nutr Cancer 62, 93.McMahon, M. et al. (2010). Biological activity of gemcitabine against canine osteosarcoma cell lines in vitro. Am J Vet Res 71, 799.Nassirpour, R. et al. (2010) Nek6 mediates human cancer cell transformation and is a potential cancer therapeutic target. Mol Cancer Res 8, 717.Oikawa, T. et al. (2010). 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品牌介绍
Anaspec阳离子抗菌肽是所有物种先天防御的重要组成部分。在许多生物中，包括真菌，昆虫，两栖动物和人类中，已经鉴定出100多种这些肽。这些疏水性和两亲性肽表现出抗生素，杀真菌，溶血，杀病毒和杀肿瘤活性。基于它们的结构特性，这些抗菌肽可分为三类：螺旋状肽，含有一个至几个二硫键的肽以及富含某些氨基酸（例如脯氨酸或色氨酸）的肽。这些肽大多数具有一些共同的特征，例如它们的低分子量（2-5 kDa），存在多个赖氨酸和精氨酸残基以及两亲性质。尽管尚不清楚它们杀死细菌的确切机制，但已表明导致膜渗透的肽-脂相互作用在其活性中起作用。抗生素肽的实例包括洋甘菊素，其由皮肤的皮肤分泌。非洲爪蟾 ; 人嗜中性粒细胞的防御素和人唾液中的组蛋白。天蚕素是由昆虫在感染条件下产生的。天蚕素A，B和D是紧密的同源物，由天蚕素蛾的35中发现的35-39个残基组成。
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