| Product Name | SensoLyte ® AFC Caspase Profiling Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-71116 |
| US$ | $698 |
Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage. They are widely used to monitor caspase activity. The SensoLyte® Caspase Profiling Kit contains a series of AFC-based peptide substrates (Ex/Em=380 nm/500 nm) as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AFC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors. The kit contains: A 96-well plate coated with a series of AFC-based caspase substrates along with various controls* Cell lysis buffer Assay buffer AFC (fluorescence reference standard for calibration) A detailed protocol A detailed protocol *384-well plate format is available on a custom basis. Kit Size: 2 plates | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C |
| Product Citations | Jaiswal, MK. et al. (2013). Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor. J Immunol 191, 5702. doi: 10.4049/jimmunol.1301604.Shteingart, S. et al. (2012). Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells. J Biol Chem 287, 31223.Lopez, V. et al. (2010). Zip6-attenuation promotes epithelial-to-mesenchymal transition in ductal breast tumor (T47D) cells. Exp Cell Res 316, 366. |
